Supplementary Figure 12: In vitro DART-Seq identifies m6A sites in cellular RNA.
From: DART-seq: an antibody-free method for global m6A detection
(a) IGV browser tracks show read coverage and C to U mutations in three representative mRNAs (JUN, HSPA5, and TMEM60; all transcribed from the negative strand). C to U mutations are indicated by gold/green (C/U) coloring, and total reads (RNA-Seq) are shown in gray. MeRIP-Seq peaks indicating regions of m6A are shown in blue. For each RNA, DART-Seq tracks from cells expressing APOBEC1-YTH or APOBEC1 alone (“In cells”; n = 3 and 2 independent samples, respectively) are shown next to DART-Seq tracks from in vitro deamination assays using HEK293T cell RNA (“In vitro”; n = 2 independent samples per condition).
