Supplementary Figure 13: Properties of in vitro DART-Seq data.
From: DART-seq: an antibody-free method for global m6A detection
(a) Metagene analysis showing a density plot of the absolute distance of C to U editing sites from the stop codon (position 0) for in vitro DART-Seq assays. C to U editing is enriched in the vicinity of the stop codon, which mirrors the distribution of m6A. n = 2 independent samples. (b) Motif analysis shows motifs found within a +/− 4 nt region surrounding C to U editing sites from in vitro DART-Seq datasets. The most enriched motif from in vitro DART-Seq assays using APOBEC1-YTH contains a GGACU sequence, which matches the m6A consensus (top; n = 9,428). In contrast, the top motif from in vitro DART-Seq assays using APOBEC1 alone fails to show enrichment for an m6A consensus motif (bottom; n = 12,206). P values were calculated using the cumulative binomial distribution.
