Supplementary Figure 15: DART-Seq estimates m⁶A abundance within RNAs.

(a) In vitro deamination assays were performed using synthetic RNAs with various amounts of m⁶A (0%, 25%, 75%, or 100%). Sanger sequencing was used to determine the relative proportion of editing at the adjacent cytidine residue. n = 2 biological replicates. (b) Quantification of the proportion of U/C editing at the cytidine adjacent to the m⁶A site in (a) reveals a direct relationship between the amount of C to U editing and the abundance of m⁶A within the RNA.