Supplementary Figure 4: Verification of safe power levels by in vitro whole-cell patch clamp recordings and trans-membrane impedance measurements in parvalbumin-positive cortical interneurons expressing tdTomato. | Nature Methods

Supplementary Figure 4: Verification of safe power levels by in vitro whole-cell patch clamp recordings and trans-membrane impedance measurements in parvalbumin-positive cortical interneurons expressing tdTomato.

From: Kilohertz two-photon brain imaging in awake mice

Supplementary Figure 4

(a) Traces of electrical activity acquired in the same neuron using +50 pA of injected current, after imaging sessions of 0, 15 and 30 min. Traces are representative of data from 4 different cells, each studied for a 30-min-duration comprising alternating 30-s-periods of imaging (1.1 mW per beamlet; 200 kHz laser emission rate) and 2-min-periods of recovery, during which we iterated through different levels of injected current (–200 pA to +50 pA in steps of 50 pA; constant current for 1 s at each level before progressing to the next). (b) Temporal widths (FWHM) of action potential waveforms for the 4 different cells over 30 min (N = 313 spikes in total). (c) Trans-membrane impedance values (N = 4 cells) across 30 min. Error bars: s.d.

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