Supplementary Figure 2: Sub-pixel alignment of STED and SMLM data.
From: A super-resolution platform for correlative live single-molecule imaging and STED microscopy
a) Images of 100 nm multicolor bead acquired in STED and SMLM after optical alignment and before sub-pixel alignment, scale bar 5 µm, 160 nm/pixel. b) Overlay between bead localizations and STED image before (green cross) and after (magenta cross) coordinate transformation. c) Histogram of pair distances before and after transformation.
