Supplementary Figure 5: STED/PALM/uPAINT correlative nanoscale imaging of spine morphology with GluA2 and PSD95 membrane proteins. | Nature Methods

Supplementary Figure 5: STED/PALM/uPAINT correlative nanoscale imaging of spine morphology with GluA2 and PSD95 membrane proteins.

From: A super-resolution platform for correlative live single-molecule imaging and STED microscopy

Supplementary Figure 5

a) Representative image of dissociated neuron imaged by correlative STED, PALM and uPAINT, scale bar 1 µm. b) Overlay of the 3 channels with a zoom on a spine, showing GluA2 AMPA receptor moving inside and outside the PSD95, scale bar 500 nm. One representative image (a, b) out of 3 independent experiments. c) Cumulative distribution of PSD95 and GluA2 AMPA receptor diffusion coefficients.

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