Supplementary Figure 9: The effect of alkaline buffer on the EM structure of GMA- and Epon-embedded samples. | Nature Methods

Supplementary Figure 9: The effect of alkaline buffer on the EM structure of GMA- and Epon-embedded samples.

From: mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM

Supplementary Figure 9

a, GMA-embedded samples without treatment. b-e, GMA-embedded samples treated with alkaline buffer with pH ranging from 7.4 to 10. f, Epon-embedded samples without treatment. g-j, Epon-embedded samples treated with alkaline buffer with pH ranging from 7.4 to 10. Scale bars, 500 nm. The experiments of a-j were performed three times with similar results.

Back to article page