Supplementary Figure 1: The fluorescence retention of cells expressing H2B-mEos4b upon osmic acid fixation.
From: mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM
a, Fixed CHO cells (4% PFA+ 2.5% GLUT) expressing H2B-mEos4b imaged by wide-filed microscopy with a 10 × air objective. b, The same filed-of-view (FOV) of a after 1% OsO4 fixation. Arrows indicating five cells with gradually decreasing fluorescence. c, The fluorescent intensity of the five cells in a before 1% OsO4 fixation. d, The fluorescent intensity of the five cells in c after 1% OsO4 fixation. Scale bar is 100 µm.
