Supplementary Figure 4: The thermal stabilities of PCFPs.
From: mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM
a, The green fluorescence thermal stability of purified mEosEM, mEos4b and intermediate mutants (~2.5µg/ml) were measured at 60 °C in Rotor-Gene 6600 real-time PCR cycler. b, The structure stability of purified mEosEM, mEos4b and other mutants with a concentration of 0.05 mg/ml in 100 mM phosphate-buffered saline (PBS, pH 7.2) measured during thermal denaturation by circular dichroism at 200 nm. The experiments of a and b were performed three times with similar results.
