Supplementary Figure 5: The osmium acid resistance of PCFPs in the green state. | Nature Methods

Supplementary Figure 5: The osmium acid resistance of PCFPs in the green state.

From: mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM

Supplementary Figure 5

a, The fluorescence intensity of PCFPs in the green state without osmium acid treatment (shaded) and with 1% OsO4 incubation for 15 min (empty). All groups have the same concentration of purified fluorescent protein (0.125 mg/ml). Bars represent mean ± s.d., n = 3 independent experiments. b, Comparison of fluorescence retention ratio of PCFPs in the green state after OsO4 treatment. Bars represent mean ± s.d., n = 3 independent experiments.

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