Supplementary Figure 9: Criteria for the FLAIR-sensitive and FLAIR-stringent isoform sets.
From: Nanopore native RNA sequencing of a human poly(A) transcriptome
a, Two candidate isoforms assembled using FLAIR. Each block represents either a complete or a partial exon (numbers 1–4). b, Reads that align to a candidate isoform. Light gray bars represent 25 nt coverage into first and last exons. c, FLAIR-sensitive isoform set that passed criteria shown at arrow. d, FLAIR-stringent isoform set that passed criteria shown at arrow. Isoform 1 failed FLAIR-stringent isoform test (X); isoform 2 passed FLAIR-stringent isoform test.
