Supplementary Figure 15: Nanopore detection of inosine base modifications.
From: Nanopore native RNA sequencing of a human poly(A) transcriptome
a, Genome browser view of a segment of the AHR gene in the GRCh38 reference. The top row shows nucleotide position and base sequence. Magenta squares (below the top row) represent putative inosine positions as characterized by RADAR1. Blue lines denote read alignments for nanopore native RNA, and brown lines denote read alignments for nanopore cDNA. White letters are mismatches relative to the GRCh38 AHR reference. White spaces with connecting black lines represent deletions in the alignment. Base miscalls occur in native RNA data at or near putative A-to-I editing sites. G base variants occur at corresponding positions in cDNA data. b, Summary of alignment information using WebLogo 2 for native RNA and cDNA data. Top row is the same ten base motif of the AHR gene as in a, with asterisks denoting putative inosines. Letter size in the logo depicts relative frequency of occurrence.
