Supplementary Figure 2: Nanopore RNA reads recapitulate known features of the human MT-transcriptome.
From: Nanopore native RNA sequencing of a human poly(A) transcriptome
a, Nanopore poly(A) RNA read coverage is consistent with the tRNA punctuation model of mitochondrial-RNA processing. Dark gray pattern represents read coverage along the heavy strand of the human mitochondrial genome. Labelled colored bars represent protein-coding genes, including known UTRs, or ribosomal RNAs. Regular font text below the colored bars identifies each gene. Yellow bars and red arrows represent the position of tRNA genes along the MT H strand. These tRNA genes are denoted by italicized text. b–d, Evidence for 3′ UTRs in MT-CO1 (b), MT-CO2 (c) and MT-ND5 (d) based on nanopore native RNA sequencing coverage. Dark gray pattern represents base coverage along specific mitochondrial transcripts. In b–d, numbering is base position relative to the human mitochondrial chromosome (chrM) for the hg38 reference. Colored horizontal lines represent protein coding sequences as in Fig. 3a of the main text. Red lines represent known 3′ UTR1. For MT-ND5, the 3′ UTR indicated by nanopore read coverage is 26 nt longer than documented1. This extension is denoted by a dashed red line in d.
