Supplementary Figure 3: Commonly observed bicistronic human MT-RNA transcripts documented by nanopore sequencing.
From: Nanopore native RNA sequencing of a human poly(A) transcriptome
a, Nanopore read-coverage plot of the mitochondrial heavy strand (gray pattern). Dotted red lines mark the predicted limits of bicistronic transcripts that encode mitochondrial ATP synthase protein 8 plus ATP synthase protein 6 (MT-ATP8/MT-ATP6), and mitochondrial NADH-ubiquinone oxidoreductase chain 4L plus NADH-ubiquinone oxidoreductase chain 4 (MT-ND4L/MT-ND4). MT-ATP8/MT-ATP6 and MT-ND4L/MT-ND4 are 841 nt and 1,667 nt long, respectively. b, Detailed view of MT-ATP8/MT-ATP6 coverage. Blue lines represent nominal length of individual gene products MT-ATP8 and MT-ATP6 within contiguous transcripts. Neighboring genes for MT-tRNA lysine (MT-TK) and Cytochrome C Oxidase Subunit 3 (MT-CO3) are marked by yellow and beige lines, respectively. c, Detailed view of MT-ND4L/MT-ND4 coverage. Blue lines represent nominal length of individual gene products MT-ND4L and MT-ND4 within contiguous transcripts. Yellow lines are neighboring genes for MT-tRNA arginine (MT-TR) and MT-tRNA histidine (MT-TH).
