Supplementary Figure 9: Three-color 4Pi-SMS images of the Golgi apparatus using cis, medial and trans markers in a HeLa cell.

(a-d) 3D images of cis Golgi (anti-GRASP65 antibody), medial Golgi (overexpressed ManII-GFP and labeled with anti-GFP nanobody), and trans Golgi (anti-p230 antibody) markers (Supplementary Video 5, part I). Merged image of the three labels in (a-c) is shown in (d) and is the same as (Fig. 3a). The size of the white 3D boundary boxes (x × y × z) is provided in the lower left corner of the images (a,d). Representative images of three independent experiments are shown (a-d).