Supplementary Figure 10: Three-color 4Pi-SMS images of the Golgi apparatus using one medial and two cis markers in a HeLa cell. | Nature Methods

Supplementary Figure 10: Three-color 4Pi-SMS images of the Golgi apparatus using one medial and two cis markers in a HeLa cell.

From: Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging

Supplementary Figure 10

(a-d) 3D overview images of cis Golgi (anti-GRASP65 antibody), cis Golgi (anti-GM130 antibody), and medial Golgi (overexpressed ManII-GFP labeled with anti-GFP nanobody) (Supplementary Video 5, part II). The size of the white 3D boundary boxes (x × y × z) is provided in the lower right corner of the images (a). (e) 1-µm thick y-z cross-section centered at the red dashed region in (d). (f) 1-µm thick x-z cross-section centered at the yellow dashed region in (d). (g) Intensity profile along the dashed line in (f). Representative images of three independent experiments are shown (a-f).

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