Supplementary Figure 1: Multicolor 4Pi-SMS microscope design and setup. | Nature Methods

Supplementary Figure 1: Multicolor 4Pi-SMS microscope design and setup.

From: Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging

Supplementary Figure 1

(a) Schematic drawing of the front of the 4Pi-SMS system. The excitation laser (green solid line) emerges from the back side of the system (IN), is reflected by the upper dichroic beamsplitter, passes through the objectives and sample, is reflected by the lower dichroic beamsplitter and returns to the back side of the system (OUT). The conventional fluorescence (red solid line) is collected by camera 1. (b) Schematic of positions and information about the dichroic beamsplitters and emission filters used in the system. (c) Photograph of the backside of the 4Pi-SMS system. The positions where the excitation laser goes to (IN) and comes back from (OUT) the front of the system are marked. The salvaged fluorescence is collected by camera 2.

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