Supplementary Figure 2: Simulation of the SF approach performance for different transition wavelengths of the dichroic beamsplitter. | Nature Methods

Supplementary Figure 2: Simulation of the SF approach performance for different transition wavelengths of the dichroic beamsplitter.

From: Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging

Supplementary Figure 2

(a) Simulation results for AF647 and CF660C, the dyes used in two-color imaging. The blue line shows the fraction of rejected molecules when 1% cross-talk is achieved. The green line shows the localization precision of AF647. (b) Simulation results for DL650 and CF680, two of the dyes used in three-color imaging. The blue line shows the fraction of rejected molecules when 2% cross-talk is achieved. The green line shows the localization precision of DL650.

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