Supplementary Figure 3: Transmission profiles for the conventional and salvaged fluorescence. | Nature Methods

Supplementary Figure 3: Transmission profiles for the conventional and salvaged fluorescence.

From: Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging

Supplementary Figure 3

(a) Transmission profiles of the dichroic beamsplitter and emission filters for the conventional fluorescence beam path. The magenta line shows the combined transmission profile of the dichroic beamsplitter and emission filters. (b) Transmission profiles of the dichroic beamsplitter and emission filters for the salvaged fluorescence beam path. The green line shows the combined profiles. (c-g) The emission spectra and the fraction of conventional and salvaged fluorescence for each dye used in this work. The blue dashed line indicates the average wavelength of each dye in the detected spectral window of the conventional fluorescence channel. The numerical value is listed in each panel. Spectra were obtained from the respective manufacturers’ websites.

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