Extended Data Fig. 1: Characterization of 1:1 standard peptide mixture.
From: Defining the carrier proteome limit for single-cell proteomics
a, The measured carrier proteome ratio vs. the number of ions (summed signal-to-noise [s/n] of all reporter ions) for each quantified spectrum. The carrier proteome ratio was calculated by dividing the sum of carrier proteome channels (TMT126 & TMT127c) by all other channels. All spectrum from (a) were ordered by descending number of ions (summed s/n) and the top 50% of the data (highest s/n) was used to create a distribution of carrier proteome ratios. The carrier proteome ratios were derived from the apex of the distribution. b, The peptide, SAAGAFGPELSR, was analyzed at 10 points across its elution. At each point ions were sampled at three AGC targets (1 × 104, 1 × 105, and 1 × 106). Individual measurements across the peak for each AGC target. For each spectrum the raw s/n is plotted. The scan(s) with the minimum CV are boxed in red.
