Extended Data Fig. 2: Design for repetitive mounting and dismounting of the FHIRM-TPM 2.0 headpiece.
From: Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging
a, Schematics of the baseplate (top) and the holder (bottom). b, Schematic illustrating headpiece mounting onto an assembly of baseplate plus a holder glued on a glass coverslip of a chronic cranial window. c, The protocol for mounting, dismounting, and remounting the headpiece. Step 1, the baseplate with a coverslip is fixed over a cranial window with dental cement. Step 2, positioning the headpiece. The headpiece is first screw-fastened to its holder and then placed onto the baseplate using a triaxial motorized stage. Once a region of interest is found, the holder is fixed to the baseplate with dental cement. Step 3, dismounting the headpiece by unscrewing and unplugging from the holder. Step 4, to remount, a drop of water is placed on the coverslip of the baseplate; then, the headpiece is plugged in and screw-fastened to the holder.
