Extended Data Fig. 3: Negligible effects of the carried headpiece on mouse movement.
From: Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging
a, Top: snapshots of a representative mouse in homecage, with the baseplate-holder assembly only (left), the assembly and a 4.2-g dummy headpiece with no fiber and cable (middle), or the assembly and the whole 4.2-g headpiece with fiber and cable (right). Bottom: mouse trajectories extracted from the videos of 30-min test sessions. Colors code the moving speeds. b, Total travel distance (left) and average speed (right) in these three conditions. Experimental results of 8 mice are presented as box-and-whisker plots, and no significant differences among three groups were found according to One-Way ANOVA test (mean ± SEM, p = 0.57 for travel distance and 0.56 for average speed). Center line, median; limits, 75% and 25%; whiskers, maximum and minimum.
