Extended Data Fig. 5: Resolution, working distance, and focusing tests of the FHIRM-TPM 2.0.
From: Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging
Agarose gel containing 400-nm fluorescent beads served as the test sample. a, xy image of the beads, averaged from 20 frames. b, xy (left) and yz imaging (right) of four fluorescent beads. Data are from a 55 × 55 × 50 μm3 z-stack data set. c, d, Normalized and averaged lateral (c) and axial (d) profiles of the four beads, yielding a full width at half-maximum of 1.13 ± 0.03 μm (c) and 12.18 ± 0.2 μm (d). The plot shows the mean ± SEM from four beads. e, Long working distance illustrated by imaging a volume of 77 × 77 × 1,100 μm3. For panels d and e, the test sample was placed on a precision Z-axis motorized stage. f, g, Volumetric reconstruction from z-stack imaging with the ETL (g) compared to that with the motorized stage (f).
