Extended Data Fig. 6: Resolving dendrites and spines with the FHIRM-TPM 2.0.
From: Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging
Images showing dendrites and spines in the frontal association cortex (FrA) of an awake, head-fixed mouse expressing GCaMP6s and bottom showing the time courses of Ca2+ changes (duration, 100 s) in four selected spines (marked with numerals in the image). Data were obtained +2.8 mm anteroposterior, +0.75 mm mediolateral at 40 μm below the pial surface. Scale bars, 30 μm (left) and 5 μm (right). Data were derived from one animal and are representative of three similar experiments.
