Extended Data Fig. 4: Characterization of EXODUS.
From: Exosome detection via the ultrafast-isolation system: EXODUS
a, Comparison of the sample processing times of EXODUS and other methods showing their detailed procedures. For each method, the time cost was basically calculated according to its protocol. b, Size distributions and c amount of the particles obtained by EXODUS and other methods from 10 mL of urine sample aliquots. (n≥5 independent experiments). d, Isolation of exosomes from 10 mL of urine sample aliquots with 20 replications to study the reproducibility of EXODUS. Qubit™ Protein Assay Kits measured the total protein amounts of isolated samples, with an average protein amount of 4.3 μg and a CV of 9.9% over the 20 measurements. e, The concentrations and f size distributions of the particles isolated from different biofluids by EXODUS. (n = 3 independent experiments). The concentration of tear exosomes is presented as particles per centimeter tear collection paper. In c and e, data are presented as mean value ± SD. NTA profiles in b and f are constructed by the average curve (solid line) and error band (shaded area).
