Extended Data Fig. 7: Cholesterol images with high intensity.
From: Mass spectrometry imaging of untreated wet cell membranes in solution using single-layer graphene
a, d, ToF-SIMS positive ion images of cholesterol at C27H45+, m/z 369.25 over areas of 500 x 500 μm2 (a) and 150 × 150 μm2 (d). b–c, e–f, Cholesterol images with pixels above a certain threshold (10 for b, c and 4 for e, f) per pixel in the images of a and d. Gradation was removed for clarity in c and f. The heterogeneous images in c and f are similar to the image reported by nano-SIMS21 using cholesterol antibodies that work for a high cholesterol concentration, which indicates that the cholesterol images in a and d show the intrinsic whole cholesterol distribution. Scale bar, 100 μm (a–c) and 30 μm (d–f). Results presented are obtained from a single experiment.
