Supplementary Figure 5: Effect of sharpening on Q-score.
From: Measurement of atom resolvability in cryo-EM maps with Q-scores
The original map (GroEL map entry 104 in 2016 EMDB map challenge) with reported resolution of 4.4Å by gold-standard FSC. The Q-score is calculated using a fitted model (PDB:4hel, 3864 atoms in chain A). Q-scores increase with sharpening (using phenix.auto_sharpen b_sharpen=B_sharpen) up to 0.31 with B_sharpen of 275, and then start to decrease with further sharpening. Visually the map has the most detail after sharpening with B_sharpen of 275, corresponding to the highest Q-score, past which it starts to become fragmented with more sharpening.
