Supplementary Figure 5: VLP Subunit Analysis. | Nature Methods

Supplementary Figure 5: VLP Subunit Analysis.

From: Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes

Supplementary Figure 5

Characterization of the capsid coat proteins (CPs) used to assemble MS2 virus-like particles analyzed in Fig. 3. The WT (top) and MINI (bottom) CP masses are within 4 part-per-million of their theoretical values. Mass measurements from denaturing the VLP capsids produced intact mass values that match and confirm the sequence differences of each species. The one differing amino acid (proline vs. serine at position 37) between the two proteoforms results in the structural differences between the WT and MINI VLPs.

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