Supplementary Figure 7: Comparison of five methods on simulated data matching 32 fibroblast cell lines and estimated error rate and cell assignability with cardelino from experimental data for 32 fibroblast lines.

(a) Assessment of cell assignment to clones across a variety of simulation settings, considering SingleCellGenotyper (SCG), Demuxlet, cardelino, cardelino-free and cardelino-fixed (Methods; Supplementary Note). Considered are simulated data based on empirical characteristics observed in 32 fibroblast lines. For each line, the sequence coverage, clone configuration (i.e., number of clones, variants on each branch), and allelic imbalance parameters were obtained to derive simulation parameters. 200 cells are synthesised per line and a guide clonal tree with 10% errors in allocation of variants to clones. (b) Estimated error rate in the clonal tree configuration derived from bulk exome-seq data (based on cardelino) for each of 32 lines versus fraction of confidently assigned cells (>90% of cells assigned for 23 lines; at cardelino posterior probability P>0.5 for most-probable clone).