Supplementary Figure 11: Direct effects of somatic variants on genes overlapping the variant.
From: Cardelino: computational integration of somatic clonal substructure and single-cell transcriptomes
Volcano plot showing negative log P values versus log2-fold change from testing differential expression for genes with a somatic mutation between cells with the mutation and cells without the mutation, faceted by VEP annotation category (Methods). Each point represents a gene, and box plots show the overall log2-fold change distribution for each annotation category. DE tests (two-sided QL F test in edgeR) are conducted within each line (donor) separately, and the results shown here are aggregated across n=32 lines. Genes are categorised by simplified functional annotations from VEP of the somatic mutation, and genes significantly DE at an FDR threshold of 20% are shown in red.
