Supplementary Figure 7: m/z position based mass prediction.
A Mass spectrum of AAV8 recorded on an QToF mass spectrometer modified for high mass measurements. Peak positions for empty and genome loaded AAV8 are indicated with empty and solid blue circles (24,012 m/z and 29,459 m/z, respectively). B Empirical dependency between m/z position and mass for 76 globular proteins, measured and reported previously, fitted with an exponential function a,b. The resulting formula Mass[kDa] = 1.63*10-6 * m/z2.14 was used to calculate the mass for the empty and genome loaded AAV8 particle (3,764 kDa and 5,828 kDa respectively). The calculated mass for the empty AAV8 is in close agreement with the expected mass but the mass of the genome loaded particle deviates by ~1 MDa. a: Veesler, D. et al. Architecture of a dsDNA viral capsid in complex with its maturation protease. Structure 22, 230–237 (2014). b: Sigmund, F. et al. Bacterial encapsulins as orthogonal compartments for mammalian cell engineering. Nat. Commun. 9, (2018).
