Supplementary Figure 1: Pressure dependent ion dephasing for the AaLS-neg nanocontainers (Mw ~ 3MDa, z ~ 145). | Nature Methods

Supplementary Figure 1: Pressure dependent ion dephasing for the AaLS-neg nanocontainers (Mw ~ 3MDa, z ~ 145).

From: Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry

Supplementary Figure 1

2D histograms for AaLS-neg ions acquired at different pressure settings before filtering. Nitrogen was used as collision gas and the readout of the ultra-high vacuum cold cathode gauge is shown, together with the percentage of ions that remain stable over the full transient, above each histogram. These stable ions are located between the dotted lines, indicating the expected intensity for ions surviving the whole set transient time of 1,024 ms. The trailing of the ion intensities towards lower values is primarily caused by peak splitting, presumably resulting from dephasing and/or mass loss of particles during the transient. Both effects are expected to originate from collisions with background gas and/or metastable decay during detection and increase at elevated pressure.

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