Supplementary Figure 6: Dynamic range in single particle experiments. | Nature Methods

Supplementary Figure 6: Dynamic range in single particle experiments.

From: Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry

Supplementary Figure 6

A 2D histogram of IgG1-RGY as shown in Supplemental Fig. 5C. To estimate the dynamic range within one experiment we compared the ion counts of the highest and lowest detectable charge states, for the highest and lowest abundant IgG oligomer, respectively. Panels B and C show an expansion of the respective region indicated with dotted lines in A. The selected charge states are shown in the 1D histogram above the expansion for B and C. Comparing the ion counts for charge state +47 of the trimeric (1536) and +49 of the tetrameric (11) IgG oligomer, yields a dynamic range of ~150, whereby the 11 ions for the charge state +49 only account for around 0.05 % of the total number of ions displayed in A.

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