Supplementary Figure 2: Experimental measurement of the point spread function (PSF) of the LaVision light-sheet Ultramicroscope II.
From: Machine learning analysis of whole mouse brain vasculature
a, Red fluorescent beads (diameter 0.1 µm) were embedded in 1% agarose gel and cleared using 3DISCO. The beads were then imaged in BABB medium (RI = 1.56) using 4× objective lens (Olympus XLFLUOR 340), at 580/25 nm excitation and with a 625/30 nm emission filter by sampling at 1.625 × 1.625 × 1 µm. b, Full width half maximum (FWHM) measure derived from the Gaussian fit to the intensity profile, along the indicated cross-sections in the center of the diffraction pattern (a) of an exemplary bead. c, Quantification of the PSF distribution (n = 6) derived from the Gaussian fittings. All data values are given as mean ± SEM.
