Extended Data Fig. 1: Properties of the hollow core photonic bandgap crystal fiber (HC-PBGF).

a, average image of pollen grains acquired from the miniature microscope using a single-ring hollow-core photonic crystal fiber with a 25 µm core diameter, showing the region of interest (ROI, green) in which the fluorescence trace in b was quantified. b, fluorescence trace before and during (gray box) bending of the fiber approximating that experienced during freely moving experiments. Insert shows a micrograph of a cross section through the fiber. c, pollen grains imaged using a HC-PBGF with a 14 µm core-diameter. ROI in which the fluorescence trace in d shown in green. d, fluorescence trace before and during (gray box) bending of the fiber. Insert shows a micrograph of a cross section through the fiber. e, spectral phase of the pulses propagating through 1.1 m of fiber, with compensation of group velocity dispersion (GVD) only (green), third-order dispersion (TOD) and GVD compensated (blue) and the flat phase corresponding to the transform-limited pulse (red) in Fig. 1d. The insert shows the same curves with magnified Y-axis scaling and the normalized spectrum used for the measurements of the spectral phases (black). f, transmission losses of the fiber as a function of wavelength. Plot shows mean (dark blue) and measurement uncertainty, calculated as described previously¹³. g, full laser pulse temporal profile after the fiber before dispersion compensation (green) and after compensation (blue) compared to calculated transform-limited pulse with the spectrum measured after the fiber (red). h, example laser spectra recorded after transmission through the HC-PBGF for incident power giving 0.5 (blue), 15.7 (red) and 87.5 mW (yellow) transmitted power at the sample. i, laser spectrum center of mass wavelength recorded after transmission through the HC-PBGF as a function of incident power (power measured at the sample). Panels a-d representative of 2 separate experiments.