Extended Data Fig. 7: Comparison of TSPO lipid structures after fitting lipids into the electron density map of TSPO structure (PDB 4UC1). | Nature Methods

Extended Data Fig. 7: Comparison of TSPO lipid structures after fitting lipids into the electron density map of TSPO structure (PDB 4UC1).

From: Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins

Extended Data Fig. 7

The FEM omit map, is shown in blue and is contoured at 1.0 s. a, shows the previous fitting where DG(16:0/18:1) was modelled (yellow sticks), b, shows PE(16:0/18:1) fitting, which is accommodated extremely well (red sticks) c, shows PG(16:0/18:1) fitting (orange sticks) with quite poor matching between the PG headgroup and electron density in that region d, displays the favourable interactions between PE (16:0/18:1) and the protein, notably the hydrogen bond between the terminal amine and the aspartic acid Asp4. Hydrophobic and hydrogen bonding interactions are indicated (green and black dots respectively).

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