Extended Data Fig. 7: RNA off-target evaluation of engineered CBEs at 72h post-transfection. | Nature Methods

Extended Data Fig. 7: RNA off-target evaluation of engineered CBEs at 72h post-transfection.

From: A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects

Extended Data Fig. 7

a, The comparison of the total number of detected RNA off-target SNVs at 72 h post-transfection. n = 6 biologically independent samples for GFP, n = 9 biologically independent samples for BE3, n = 7 biologically independent samples for BE3R126E and n = 2 biologically independent samples for YE1-BE3 groups. All values are presented as mean ± s.e.m. P values above each bar were calculated by comparing with GFP group with two-sided Student’s t-tests. b, The distribution of mutation types for GFP, BE3, and BE3 variants-treated groups. c, Editing rate of RNA off-targets for BE3 variants at 72 h post-transfection.

Back to article page