Extended Data Fig. 3: On-target editing efficiency and characteristics of off-target SNVs of engineered CBEs. | Nature Methods

Extended Data Fig. 3: On-target editing efficiency and characteristics of off-target SNVs of engineered CBEs.

From: A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects

Extended Data Fig. 3

a, On-target editing efficiency of BE3 and CBE variants from WGS data. Two BE3 embryos without sgRNAs were not shown as they have no target site. b, Comparison of C-to-T and G-to-A conversions between CBE variants-treated and Cre or BE3 groups. n = 3 biologically independent samples for Cre, n = 6 biologically independent samples for BE3, n = 12 biologically independent samples for BE3R126E, n = 3 biologically independent samples for BE3R132E, n = 8 biologically independent samples for YE1-BE3, and n = 3 biologically independent samples for FE1-BE3. Two Cre samples and six BE3 samples were derived from Zuo et al.22 and one Cre sample was newly generated in this study. All values are presented as mean ± s.e.m. P value was calculated by two-sided Student’s t-test.

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