Extended Data Fig. 8: Angles from 36plex.
From: 3D mapping and accelerated super-resolution imaging of the human genome using in situ sequencing
a, Measurements of angles formed by three points along the p arm (left), q arm (right), and intersection of vectors formed by pR2-pR3 and qR1-qR2 (middle) for each chromosome. Measurements were obtained by combining data from 36plex-5K and 36plex-1K analyses and selecting chromosomes that had all six targets identified. Chr2: n = 686, Chr3: n = 668, Chr5: n = 363, Chr16: n = 586, Chr19: n = 760, ChrX: n = 493 (n = 1,051 cells from 24 replicates; for 36plex-5K, n = 611 from 15 replicates; for 36plex-1K, n = 440 from 9 replicates). b, Distribution of angles formed by segments in panel a. The thick line in each violin plot represents the Interquartile range (IQR), the white dot marks the median and the thin lines extend 1.5 times the IQR. c, Box plots comparing p and q arm angles. Two-sided student’s t-test with null hypothesis of equal mean was performed to compare arms, ns p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Boxes represent the IQR (25th, 50th and 75th percentiles) and whiskers extend 1.5 times the IQR. Sample size information in a). Exact p-values for each chromosome: Chr.2 = 4.149e-16, Chr.3 = 0.004, Chr.5 = 0.093, Chr.16 = 1.357e-14, Chr.19 = 3.325e-11, Chr.X = 0.101. d, Linear least-squares regression between arm angle and arm length with Pearson correlation coefficient r = 0.26 and p-value = 0.42 (two-sided, using slope = 0 for null hypothesis and Wald Test with t-distribution as test statistic).
