Extended Data Fig. 4: Synthesis and spectral properties of 15–20.
From: A general method to optimize and functionalize red-shifted rhodamine dyes

a, Synthesis of Janelia Fluor dyes 15–20. b–g, Fluorescence excitation (ex) and emission (em) spectra of JF669 (15; b), JF690 (16; c), JF722 (17; d), JF724 (18; e), JF571 (19; f), and JF593 (20; g).