Extended Data Fig. 5: Specific distributions of AuNPs in the E. Coli cells expressing MBP-MTn-FliG.
From: Genetically encoded tags for direct synthesis of EM-visible gold nanoparticles in cells
a, Hardly seen any AuNPs in the wild-type BL21(DE3) cells; b, Relatively uniform distribution of AuNPs in a cross-section of a E. coli cell expressing MBP-MTn-FliG. c, AuNPs accumulated at the pole of an E. coli cell that expressed MBP-MTn-FliG, only a few AuNPs found in the nucleoid region, but no AuNPs found in the periplasmic spaces (pointed by the black arrows). Specimen prepared with ‘-60˚C MeOH’ fixation followed with 2-ME/D-P AuNP synthesis. Representative images for (a-c) were selected from n = 10 independent experiments with similar results.
