Extended Data Fig. 7: An overview image showing the specific distribution of GFP-MTn-KDEL molecules in a HeLa cell treated with Scheme 3c (HPF/FSF).
From: Genetically encoded tags for direct synthesis of EM-visible gold nanoparticles in cells
An EM image of a 90 nm thick section of a GFP-MTn-KDEL expressing cell showing an overview of the ultrastructural preservation as well as the highly specific localization of the AuNPs synthesized on the fusion protein molecules accumulated in ER lumens, almost no AuNPs in mitochondria (M), multivesicular bodies (MVB), cytosol and nucleus. A high magnification image of the boxed region showing more details (Supplementary Fig. 17H(a)). Representative image was selected from n = 3 independent experiments with similar results.
