Extended Data Fig. 10: Subcellular distribution patterns of AuNPs synthesized on cysteine-rich tags in cells.
From: Genetically encoded tags for direct synthesis of EM-visible gold nanoparticles in cells
The chart is showing the statistical distribution patterns of the average AuNPs densities (AuNP counts per 0.25 µm2 obtained from 90nm-thick sections) in various subcellular organelles in S. pombe cells or HeLa cells expressing cysteine-rich tags (MTn or AFP). The averaged AuNPs densities in endoplasmic reticulum (ER), cytosol, nuclei (Nu), lysosomes (Lyso), multivesicular bodies (MVB) and mitochondria (Mito) were obtained from 19-26 EM images of 90n-thick sections of cells (Supplementary Note 15). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s multiple comparisons using GraphPad Prism 8.4.2 software. Quantitative data were expressed as box-and-whisker plots (center line, average; limits, 75% and 25%; whiskers, maximum and minimum). The specimens in the left column (a, c, e, g) were prepared with Scheme 2b or 2d (using a strategy of oxidization and G A fixation). The specimens in the right column (b, d, f, h) were prepared with Scheme I (-60 °C MeOH) or Scheme 3c (HPF/FSF) (using a rapid freezing strategy without using oxidization and GA fixation) (Methods). The averaged densities of AuNPs in different organelles demonstrated that the tags had significantly specific localizations to the targeted organelles, while the background noises were quite low (< 2-5%). All the P values as shown in (a-h) are: P < 0.0001 (95% CI). The numbers of EM images used for (a-h) are: (a) n = 20; (b) n = 19; (c) n = 23; (d) n = 22; (e) n = 19; (f) n = 22; (g) n = 22; (f) n = 26. Size (n) for each organelle is labeled on the figure.
