Extended Data Fig. 4: Negative controls for CIT-induced ER-mitochondria-PM tri-organellar membrane contact sites (MCS).
From: Rational design and implementation of a chemically inducible heterotrimerization system
a, Assessing contributions of each CIT component in tri-organellar MCS formation; left, middle and center panels correspond to constructs lacking ER, mitochondria, and PM CIT components. b, Quantifying trimerization between the 3 signals pre- and post- 100 nM rapamycin. Check marks specify each combination of two wavelengths used in calculating pairwise Fisher’s transformation of Pearson’s correlation coefficients. Data are presented as mean values +/- SD. Two-tailed Student’s t-test assuming equal variance was used to compare correlations pre- and 15 mins post-rapamycin. From left to right: n = 24, 32 and 28 cells; 3 independent experiments each. Scalebar, 10 μm. (****; p < 0.0001).
