Extended Data Fig. 5: PIP2 biosensor intensity at ER-PM junction sites over time.
From: Rational design and implementation of a chemically inducible heterotrimerization system
Intensities of mRuby–PH-PLCδ PIP2 biosensor of user defined regions of interest inside and outside ER-PM MCS at 1, 2, 3, 4 and 10 min after 100 nM rapamycin. Significance analyzed with two-tailed paired Students t-tests assuming equal variance. From left to right: n = 28, 25 and 28 cells; 4 independent experiments each. (*/***/****; p < 0.05/0.001/0.0001).
