Extended Data Fig. 3: AC-mito expression does not alter endogenous Fis1 localization to mitochondria.
From: Actin chromobody imaging reveals sub-organellar actin dynamics
Maximum projection of cells expressing AC-mito and non-transfected cells show similar levels of endogenous anti-Fis1 immunofluorescence. Note that the anti-Fis1 antigen is the Fis1 N-terminus, which is not present in the AC-mito protein, which contains only the C-terminus of Fis1. Scale bar: 20 µm. These results were reproducible across 2 independent experiments.
