Extended Data Fig. 4: Alternate membrane-targeting and actin-binding motifs yield similar results.
From: Actin chromobody imaging reveals sub-organellar actin dynamics
Switching the actin nanobody motif with LifeAct (Fis1-LifeAct-GFP) yields similarly increased accumulation compared to control constructs as quantified by the coefficient of variance. Switching the Fis1 mitochondrial outer membrane targeting sequence for Cytb5mito (Cytb5mito-AC-GFP) also yields similar results. Scale bar: 5 µm. The table displays the degree of accumulation as quantified by the average coefficient of variance (standard deviation/mean pixel intensity in the mitochondrial region) ± the standard deviation for each probe compared to control probes. N indicates the number of cells analyzed. P-values determined by two-tailed Welch’s t-test. These results were reproducible across 7 independent experiments.
