Extended Data Fig. 8: Recording of ACh signal during sleep-wake cycle.
From: An optimized acetylcholine sensor for monitoring in vivo cholinergic activity
a, The schematic illustration and representative recording data of ACh3.0-mut sensor during the sleep-wake cycle in mice. b, The group data of the fluorescence response of ACh3.0 and ACh3.0-mut sensors in different sleep-wake status (n = 5 mice for ACh3.0 and n = 6 mice for ACh3.0-mut, p=0.0009 in wake; p=0.049 in NREM; p=0.008 in REM). c, The representative recording data of ACh3.0-mut sensor during the sleep-wake cycle in mice. Similar results as the representative images were observed for 6 mice. d, Multiple recording traces of the ACh3.0 sensor during the sleep-wake cycle (from 3 mice, additional to the representative one in Fig. 3g). All data are shown as mean value ± SEM, with the error bars or shaded regions indicating SEM. Two-sides Student’s t test performed in (b); *, p<0.05; **, p<0.01; ***, p<0.001.
