Extended Data Fig. 9: Imaging of ACh signal in the cortex.
From: An optimized acetylcholine sensor for monitoring in vivo cholinergic activity
a, Cartoon illustration of the miniature two-photon microscope. b, Group data of ACh3.0 fluorescence in mice recorded while running on a treadmill at indicated speeds (n = 5 mice). c, Representative traces and group summary of ACh3.0 fluorescence measured in mice while performing the running task; where indicated, the mice receive an i.p. injection of saline (black), the nAChR blocker mecamylamine (Meca, 2 mg/kg body weight, blue), or the M3R antagonist scopolamine (Scop, 20 mg/kg body weight, dark yellow); each trace is averaged from 10 trials; n = 5 mice per group, p=0.54 between Saline and Meca; p=0.0002 between Meca and Scop. d, Pseudocolor images showing the ACh3.0 fluorescence response in the S1 in the Hit trial of the whisker-guided object location discrimination task. The left, middle and right image showing the response during baseline, peak in the answer period and after response. Similar results as the representative images were observed for 3 mice. Scale bar: 100 μm. All data are shown as mean value ± SEM, with the error bars or shaded regions indicating SEM. Two-sides Student’s t test performed in (c); *p<0.05, **p<0.01, ***p<0.001, and n.s., not significant.
