Extended Data Fig. 1: Fluorescence decays and exponential fits of Cy5 before and after irradiation.
From: Photoblueing of organic dyes can cause artifacts in super-resolution microscopy
The photoconverted (photobleached) product was purified by HPLC. The fluorescence decays were recorded at the emission maxima of 670 and 565 nm, respectively, in PBS, pH 7.4. The lower panels show residual differences between experimental and fitted values. We measured main lifetime components of 0.93 ns with an amplitude > 98% before irradiation and 0.32 ns with an amplitude > 98% for the photoconverted product.
